Two Research Assistant positions in the group of Dr Marko Hyvonen at the Department of Biochemistry, University of Cambridge, Cambridge have arisen (http://www.bioc.cam.ac.uk/people/uto/hyvonen) to support the group leader and the research group in protein expression and purification, in molecular biology and in the general running of the laboratory.
Applicants are expected to have experience in protein production and/or modern molecular biology techniques. In protein production the candidates should be familiar with large scale protein expression in bacteria (experience in yeast or mammalian cells an additional advantage), refolding and purification using automated chromatography systems (GE AKTAs) and in general handling of proteins and other macromolecules. Experience in working with disulfide-linked proteins and purification of challenging protein using reverse phase chromatography is highly desired. In molecular biology it is essential to have experience in all stages of expression construct and primer design, PCR, restriction-based and ligation independent cloning, plasmid preparation and sequence analysis. Familiarity with molecular biology software and management of plasmid/clone databases would be an additional advantage.
Applicants with previous experience from structural biology laboratories and protein crystallisation are highly desired.
The post holders will be jointly in charge of the general running and coordination of the daily activities of a research group of 10+ people, and in charge of ordering of chemicals and consumables. They will also advise the group leader in the maintenance and purchase of equipment and they will support the research effort of individual researchers and students in the group.
The applicants are expected to have good communication skills, to work well in a collaborative environment within the research group and with collaborators. The applicants should be pro-active individuals with great attention to detail and able to produce high quality work and report the results back to the group leader. Good IT skills are essential. We seek candidates with a degree in a biological science.
Informal enquires can be directed to Marko Hyvonen - email@example.com
Fixed-term: The funds for this post are available for one year in the first instance.
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