Applications are invited for Addenbrooke's Charitable Trust (ACT) and Bascule Charitable Trust funded Junior Research Laboratory Technician.
The technician will work at the Clifford Allbutt Building - Cambridge Biosciences Campus and join the Pluchino laboratory (www.pluchinolab.org), whose research in the areas of Neuroinflammation and Stem Cell Neurobiology includes the development of personalised molecular therapies (stem cells and gene delivery) aimed at providing neuroprotection in chronic neurological illnesses among which progressive multiple sclerosis. The Pluchino laboratory is strategically located within the scientific environment of the Addenbrooke's hospital area. There are ample opportunities to interact with other laboratories with interests in regulation of gene expression and disease. The Department of Clinical Neurosciences has a strong emphasis on development of novel tools for brain and axonal regeneration using medical devices, gene vectors and stem cells.
The candidate should have previous experience in a laboratory and great organisational skills. Experience with cell culture techniques (standard propagation of cell lines and stem cells, freezing and waking-up cells stocks, in vitro transformation assays), molecular biology techniques (PCR, genotyping, RT-PCR, nucleic acids and protein extraction, Western Blot analysis) and immunofluorescence is warranted. Experience with viral vector production/titration and/or holding a personal license (PIL) to carry out animal research is desirable. The scope of the role is to help the scientists with in vitro (and possibly in vivo) work aimed at understanding disease pathogenesis in disease models of neurodegenerative/immunological disorders, as well as applying strategies for CNS repair (e.g. via systemic or local delivery). The chosen candidate will be responsible for preparing cell culture media and solutions, making aliquots, propagating cell lines (+ freezing/thawing), managing liquid nitrogen tank, sending samples for mycoplasma testing, and help with in vivo work (genotyping and handling of transgenic mouse colonies, disease induction, behavioural tests, collection and processing of tissues/histology). Organisational skills will be needed to manage cell archives, check and order lab supplies, storing lab reagents, update reagents databases and help with national/international shipments. The Principal Investigator may also from time to time require the role holder to perform other duties appropriate to the grade of the role.
Applicants should have good inter-personal and communication skills, being a team player, having strong organisation skills, as well as proficient knowledge of English.
Relevant Publications 1. Smith JA, et al. RNA Nanotherapeutics for the Amelioration of Astroglial Reactivity. Mol Ther Nucleic Acids. 2018 Mar 2;10:103-121; 2. Peruzzotti-Jametti L, et al. Macrophage-Derived Extracellular Succinate Licenses Neural Stem Cells to Suppress Chronic Neuroinflammation. Cell Stem Cell. 2018 Mar 1;22(3):355-368; 3. Iraci N, Gaude E, et al. Extracellular vesicles are independent metabolic units with asparaginase activity. Nat Chem Biol. 2017 Sep;13(9):951-955.
Fixed-term: The funds for this post are available until 31 August 2019 in the first instance.
Once an offer of employment has been accepted, the successful candidate will be required to undergo a health assessment and a security check.
Informal enquiries should be addressed to Dr Stefano Pluchino on email@example.com
To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.
Closing date: 26 August 2018
Interview Date: likely to be 28-29 August 2018
Please ensure that you outline how you match the criteria for the post and why you are applying for this role on the online application form.
Please include details of your referees, including e-mail address and phone number, one of which must be your most recent line manager.
Please quote reference XB16405 on your application and in any correspondence about this vacancy.
The University values diversity and is committed to equality of opportunity.
The University has a responsibility to ensure that all employees are eligible to live and work in the UK.