Applications are invited for a full-time Research Assistant in the laboratory of Professor Steve Jackson (http://www2.gurdon.cam.ac.uk/~jacksonlab/). The main lab is based in the internationally renowned Gurdon Institute in the centre of Cambridge. The candidate will be part of the satellite lab based at the Sanger Institute, Hinxton but will be required to attend the main lab for weekly meetings, and on other days as required. The successful candidate will carry out cell and molecular biology project work under the supervision of an experienced postdoctoral researcher, which requires highly skilled technical knowledge and competence, as part of a large research group working on the structure and function analysis of DNA repair. Core duties will involve stable cell line generation, knockout cell line generation using CRISPR/Cas9 technology, routine tissue culture, cloning and other molecular work, as well as biochemistry, including protein purification. In addition, while the post does not involve any animal handling, a background in mouse colony management is desirable.
Other responsibilities will include preparation of reagents, maintenance of lab stocks and assisting in the day-to-day running of the laboratory.
Preferred skills and experience:
- Degree or equivalent in life sciences
- Previous research laboratory experience
- Mammalian tissue culture, significant experience required (including transfection, siRNA and stable cell line generation)
- Previous significant experience in molecular biology techniques: cloning, PCR, mutagenesis, western blotting, DNA, RNA and protein analysis, and protein purification or immunofluorescence
- Experience in CRIPSR/Cas9 technology preferred
- FACs and/or fluorescence microscopy experience preferred
- High attention to detail and good record keeping
- Good communication and organisational skills
- Reliability and enthusiasm
Starting date: As soon as possible. Please note that applicants may be invited for interview prior to the closing date.
Limited funding: The funds for this post are available for 2 years in the first instance.
To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.
Please ensure that you upload your Curriculum Vitae (CV) and a brief statement outlining your research interests in the Upload section of the online application. If you have any questions about this vacancy or the application process, please email email@example.com. Please quote reference PR14901 on your application and in any correspondence about this vacancy.
The University values diversity and is committed to equality of opportunity. The University has a responsibility to ensure that all employees are eligible to live and work in the UK. Benefits include generous maternity/paternity leave, flexible working, funds for returning carers and other family-friendly schemes.
The Gurdon Institute is committed to equality and diversity in the workplace, for more information go to https://www.gurdon.cam.ac.uk/about/athenaswan.