The Waller laboratory based in the University of Cambridge, Department of Biochemistry is looking to recruit a Senior Research Technician.
The position will support a research project investigating the cell biology of apicomplexan parasite Toxoplasma gondii. This project entails screening large numbers of parasite proteins for cell location and function. The technician will assemble, prepare and quality control new expression, knockdown and knockout DNA constructs, transfect and clone the new parasite cell lines and prepare media and other reagents required for this project. The technician will also be trained to assist with preliminary screening of new transfectant lines, by immunofluorescence assay, Western blotting and genomic PCR, where their time allows.
Key duties: - cell culture of human and parasite cell lines - prepare media and other chemical reagents - basic assembly of DNA constructs including PCR, plasmid DNA purification and sequencing - assist with parasite transformation and primary analysis of these new cell lines.
The successful candidate should have a HNC level qualification or equivalent experience. Experience with Toxoplasma culturing and transfection, Golden Gate cloning methods, and fluorescence microscopy will be a strong advantage.
Fixed-term: The funds for this post are available for up to 12 months in the first instance.
For all informal inquiries, please contact Dr Ross Waller - firstname.lastname@example.org
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Please quote reference PH12778 on your application and in any correspondence about this vacancy.
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