Applications are invited for a Research Associate position within a Human Frontier Science Program-funded collaborative project between Nick Brown in Cambridge and Gregory Giannone in Bordeaux. This project aims to extend the boundaries of single-molecule imaging to examine live protein interactions within adhesion structures in the intact, developing organism, using Drosophila as our model. Furthermore, using the latest in optogenetic techniques we will examine how altering interactions within integrin complexes impacts on their function within the organism.
We are seeking a skilled molecular biologist to build Drosophila strains containing integrin-associated proteins tagged with fluorescent proteins suitable for super-resolution imaging and single-particle tracking. This will be accomplished by a combination of standard transgenesis and CRISPR/Cas9-induced repair. These strains will be imaged using state of the art microscopy by our collaborators at the Interdisciplinary Institute for Neuroscience in Bordeaux, to reveal how protein interactions within the complex alter molecular behaviour. Armed with this knowledge we will manipulate these interactions to discover how they contribute to the morphogenetic and developmental functions of integrin adhesion.
Applicants should have, or be close to finishing, a PhD degree in a relevant subject. The successful candidate will be enthusiastic about molecular biology and already have a strong track record in building complex recombinant DNA constructs. Experience in any of these fields will also be advantageous: cell adhesion, cytoskeleton, biophysics, generating new fluorescent proteins/biosensors, advanced microscopy, and genetics and development of Drosophila. The post is available immediately.
Fixed-term: The funds for this post are available for 3 years in the first instance.
To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.
Applicants should provide a covering letter describing their highest molecular biology achievement and a CV and contact details of three referees. Informal enquiries may be made to Professor Nick Brown (firstname.lastname@example.org).
The closing date for applications is midnight on Wednesday 2 August 2017.
Please quote reference PM12585 on your application and in any correspondence about this vacancy.
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