Fixed-term: The funds for this post are available for 1 years in the first instance.
Applications are invited for a Research Assistant role in the Centre for Misfolding Diseases to assist in studies related to the development of a real-time imaging cell model of TDP-43 towards understanding how the trafficking of this protein relates to Motor Neurone Disease (MND). The project involves producing transfected mammalian cell lines over-expressing TDP-43 and monitoring the translocation of the protein using immunohistochemistry and confocal microscopy.
The successful candidate will use transient transfection techniques (lipofection, Neon transfection system) to introduce TDP-43 and mutational variants to mammalian cell lines. Using these cell lines, the successful candidate will image the protein over time and use immunohistochemistry to perform co-localisation experiments with a variety of biological markers. Real time imaging using bisarsenical dyes will be performed to follow the real-time translocation and trafficking of the protein and biochemical analysis of cell lysates, including immunoprecipitation and western blotting techniques will be used to fully characterise the model. Other duties may include, but are not limited to, recombinant expression of TDP-43 variants in E.coli and protein purification (affinity and size-exclusion chromatography) and site-directed mutagenesis.
The successful candidate will have a B.Sc degree (or equivalent) in a relevant biological science. A fundamental background in cell biology is essential. They will be highly motivated, have excellent organisational skills and be able to work independently. The successful candidate will have experience in tissue culturing techniques, immunohistochemistry and confocal microscopy imaging. They will be familiar with DNA plasmid purification and site-directed mutagenesis. Some experience with recombinant protein expression and purification is desirable but not essential. Knowledge of real-time confocal microscopy would be an asset.
To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.
Please ensure that you upload your Curriculum Vitae (CV), a covering letter and publications list in the upload section of the online application. If you upload any additional documents that have not been requested, we will not be able to consider these as part of your application.
For more information about the post please contact Dr. Janet Kumita (firstname.lastname@example.org).
Please quote reference MA12331 on your application and in any correspondence about this vacancy.
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