DNA repair is crucial in all living cells for the maintenance of genome stability. The majority of spontaneous DNA lesions arise from the intrinsic chemical instability of DNA and include thousands of DNA single-strand breaks (SSBs) that occur on a daily basis. If left unrepaired, SSBs can terminate gene transcription, leading to mutations, cell death and disease, including premature ageing, cancer and progressive neurodegeneration.
We are looking to recruit a highly motivated and enthusiastic Postdoctoral Research Associate to investigate the novel functions of the ATM kinase in SSB repair (Khoronenkova & Dianov, PNAS, 2015; Khoronenkova et al., Mol Cell, 2012). We will determine the molecular mechanisms by which ATM is activated by SSBs. We will also investigate whether SSB repair deficiencies are linked to the genetic instability and neurodegenerative phenotypes of Ataxia Telangiectasia, caused by mutations in the ATM gene. The project will use biochemical in vitro assays and in vivo targeting of DNA nucleases to defined genomic loci, patient-derived induced pluripotent stem cells, CRISPR/Cas9 editing, confocal microscopy and state-of-the-art biochemical and proteomic approaches.
The post holder will join the laboratory of Dr Svetlana Khoronenkova at the Department of Biochemistry, University of Cambridge, United Kingdom.
Applicants should have a PhD (DPhil) in Biochemistry or Molecular/Cell Biology and a strong biochemical background. Previous experience in the fields of stem cell biology and/or DNA repair would be an advantage.
Fixed-term: The funds for this post are available for 36 months in the first instance.
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For informal enquiries please contact Dr Svetlana Khoronenkova at email@example.com.
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Please quote reference PH11985 on your application and in any correspondence about this vacancy.
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