Applications are invited for a full-time Research Assistant in the laboratory of Professor Steve Jackson (http://www2.gurdon.cam.ac.uk/~jacksonlab/) based in the internationally renowned Gurdon Institute in the centre of Cambridge. The successful candidate will carry out cell and molecular biology project work under the supervision of an experienced postdoctoral researcher. The project will involve identifying genetic components in DDR-deficient cells that confer resistance/and or sensitivity to a defined drug portfolio. Core duties will involve stable cell line generation, knockout cell line generation using CRISPR/Cas9 technology, routine tissue culture, cloning and other molecular work, as well as biochemistry, including protein purification.
Other responsibilities will include preparation of reagents, maintenance of lab stocks and assist in the day-to-day running of the laboratory.
Preferred skills and experience:
- Degree or equivalent in life sciences
- Previous research laboratory experience
- Mammalian tissue culture, significant experience required (including transfection, siRNA and stable cell line generation)
- Previous significant experience in molecular biology techniques: cloning, PCR, mutagenesis, western blotting, DNA, RNA and protein analysis, and protein analysis or immunofluorescence
- Experience in CRIPSR/Cas9 technology is required
- FACs and/or fluorescence microscopy experience preferred
- High attention to detail and good record keeping
- Good communication and organisational skills
- Reliability and enthusiasm
Starting date: 1 June 2017 or as soon as possible after this date.
Limited funding: The funds for this post are available until 31 May 2019 in the first instance.
To apply online for this vacancy, please click on the 'Apply' button below. This will route you to the University's Web Recruitment System, where you will need to register an account (if you have not already) and log in before completing the online application form.
Please ensure that you upload your Curriculum Vitae (CV) and a brief statement outlining your research interests in the Upload section of the online application. If you have any questions about this vacancy or the application process, please email email@example.com. Please quote reference PR11958 on your application and in any correspondence about this vacancy.
The University values diversity and is committed to equality of opportunity. Benefits include generous maternity/paternity leave, flexible working and funds for returning carers and other family-friendly schemes. The University has a responsibility to ensure that all employees are eligible to live and work in the UK.